Office of Undergraduate Instruction

01:694:313 Introductory Biochemistry Laboratory

NOT for students majoring in Molecular Biology and Biochemistry.

This course may be used to fulfill the elective requirements and laboratory requirement of the Biological Sciences major.


Offered:  Fall, Spring, Summer

Credits:  1

Prerequisites:  Organic Chemistry (01:160:307) and  Biochemistry  (01:694:301, 11:115:301, or 01:694:407)

Course Description:  This course provides familiarization with protein and enzyme techniques in the laboratory, and properties of amino acids and sugars. There is some material dealing with DNA and very basic molecular biology. The lab complements the material in the course, 694:301, but is not taught in parallel with 301.

Course URL:  

Course satisfies Learning Goals

1. Students should demonstrate an understanding of the knowledge that is needed to begin biomedical research and that is required for post-graduate exams and studies.

3. Students should demonstrate the ability to design experiments and critically analyze data.

Week   Topic

 1

2

3

4

5

6

7

8

9

10

11

12

13

14

15

 Lab 1 Course Introduction, Pipeting Exercises

Lab 2 DNA Concentrations, Plate Cells

Lab 3 Photometry, Set up Bacterial ON Cultures 

Lab 4 Plasmid Purification

Lab 5 PCR/ Restriction Enzymes 

Lab 6 DNA Gel Electrophoresis

Lab 7 Bioinformatics - DNA Sequence Analysis

Common Mid-Term Exam No labs

Lab 8 Induce protein expression 

Lab 9 Chromatography Nickel Column 

Lab 10 Protein Concentration Assay

Lab 11 Protein Gel Electrophoresis

Lab 12 Galactosidase Km, Galactosidase Inhibition

Lab 13 Protein Structure Analysis

Common Final Exam 

Exams, Assignments, and Grading Policy:  Grade is based on exams (50%), lab reports (40%), performance in lab, and attendance (10%). A laboratory course can't be taken in absentia, you must show up and participate in running the experiments.

Course Materials

Required text: Online notes posted on Sakai

Course Closed?  There is only limited space in the laboratory so No special permission numbers will be administered for this course. If the course is closed you should continue to check WebReg to see if a spot opens.

Faculty

Dr. Andrew Vershon
Waksman Institute, Rm 231
phone: (848) 445-2905
email: This email address is being protected from spambots. You need JavaScript enabled to view it.

Office Hours: Tuesday 8:00 -10:00AM


** All information is subject to change at the discretion of the course coordinator.

 

Contact Us

Nelson Biological Laboratories

Nelson Biological Laboratories
604 Allison Rd
Piscataway, NJ 08854


p. (848) 445-2075
f.  (732) 445-5870