Office of Undergraduate Instruction

 

This course is intended for undergraduate Molecular Biology and Biochemistry (MBB) majors or those with a strong background in the Biological Sciences. It may be used to fulfill the elective requirements of Biological Sciences or MBB majors.


Offered: Spring

Credits:  3

Prerequisites: Molecular Biology and Biochemistry 01:694:407 and Genetics 01:447:380

Course Description

This course is a survey of modern techniques of protein biochemistry, bioinformatics, proteomics, and functional genomics. It will include a discussion of basic concepts of protein structure and function, protein characterization and purification, enzyme kinetics, nmr and x-ray crystallography, mass spectrometry, and various techniques of functional and structural genomics.

1          Course introduction and overview of genomics & proteomics           

2          New strategies and technologies for DNA sequencing                      

3          DNA sequencing – current technologies & applications                    

4            Applications of new DNA sequencing technologies (con’d)             

5          Amino acids and proteins                                                                   

6          Conformational properties of proteins                                    

7          Blast and dynamic programming algorithm                            

8          Sequence-based bioinformatics                                                          

9          Analytical and preparative protein chemistry                                      

10        Preparative protein chemistry (cont’d)                                    

11        Protein domains and folds                                                                  

Take-home Exam I handed out

12        Structural Bioinformatics                                                                   

13        Protein folding in vitro                                                                       

Take-home Exam I due

14        Protein folding in vivo                                                                        

15        Protein NMR and structure analysis                                                  

16        Protein X-ray crystallography                                                            

17        Protein Mass Spectrometry and Proteomics                                       

18        Cryo Electron Microscopy.  Homology Modeling                             

19        Protein-protein interactions I                                                              

20        Protein-protein interactions II                                                             

21        Enzyme kinetics                                                                                 

22        Enzyme kinetics / Protein dynamics                                                   

Take-home Exam II handed out

23        Student Presentations                                                                         

24        Student Presentations                                                                         

Take-home Exam II due

25        Student Presentations                                                                         

26        Student Presentations                                                                         

27        Student Presentations                                                                         

28        Student Presentations                                                                                     

Take-home Exam III

 

Course URL: All course materials will be posted on Sakai

Course satisfies Learning Goals

1. Students should demonstrate an understanding of the knowledge that is needed to begin biomedical research in the areas of proteomics, functional genomics, and/or bioinformatics. They will learn the theory and practice of key methods needed for postgraduate studies in these fields. .

2. Students should demonstrate the ability to work independently to find and evaluate information about specific biological systems or problems.

 Exams, Assignments, and Grading Policy

Take-home Exams I & II will each count for 30% of your grade.  The Student Presentation counts for 25% of your grade.  Take-home Exam III counts for 15% of your grade.  We also take into account class participation, especially in the Student Presentation part of the course.  There will be no final exam.

Course Materials:  Readings provided by instructors.

Course Closed?

This course is by special permission only, with priority given to MBB majors. The majority of the class will be juniors and seniors. To obtain a special permission number or to get on the waiting list if you are not an MBB major, please use the following link:Wait List Sign Up for Spring 2018 Courses If you have any questions, please contact the Division of Life Sciences - Office of Undergraduate Instruction at 848-445-2075 or visit our office at Nelson Biological Laboratories B112, Busch Campus.

Faculty

Prof. Gaetano Montelione
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Prof. Stephen Anderson
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** All information is subject to change at the discretion of the course coordinator.

 

Contact Us

Nelson Biological Laboratories

Nelson Biological Laboratories
604 Allison Rd
Piscataway, NJ 08854


p. (848) 445-2075
f.  (732) 445-5870